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T2 Magnetic Resonance for Fungal Diagnosis

  • Fainareti N. Zervou
  • Ioannis M. Zacharioudakis
  • Jaclynn Kurpewski
  • Eleftherios Mylonakis
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1508)

Abstract

Rapid diagnostic methods for fungal infections are long awaited and are expected to improve outcomes through early initiation of targeted antifungal therapy. T2Candida panel is a novel qualitative diagnostic platform that was recently approved by the US Food and Drug Administration (FDA) for diagnosis of candidemia with a mean time to species identification of less than 5 h. T2Candida panel is performed on the fully automated T2Dx instrument in whole blood K2EDTA specimens and is able to detect 5 Candida spp., namely Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, and Candida glabrata. By combining magnetic resonance with molecular diagnostics, T2Candida panel amplifies DNA and detects the amplified product by amplicon-induced agglomeration of supermagnetic particles and T2 Magnetic Resonance (T2MR) measurement. Here we describe the materials and methods needed to diagnose candidemia with the T2Candida panel.

Key words

T2Candida panel T2 magnetic resonance T2MR Candida 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Fainareti N. Zervou
    • 1
  • Ioannis M. Zacharioudakis
    • 1
  • Jaclynn Kurpewski
    • 2
  • Eleftherios Mylonakis
    • 1
  1. 1.Infectious Diseases Division, Rhode Island HospitalWarren Alpert Medical School of Brown UniversityProvidenceUSA
  2. 2.Division of Infectious Diseases, Department of Medicine, Miriam Hospital/Alpert Medical SchoolBrown UniversityProvidenceUSA

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