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Microencapsulation of Hepatocytes and Mesenchymal Stem Cells for Therapeutic Applications

  • Raphael P. H. Meier
  • Elisa Montanari
  • Philippe Morel
  • Joël Pimenta
  • Henk-Jan Schuurman
  • Christine Wandrey
  • Sandrine Gerber-Lemaire
  • Redouan Mahou
  • Leo H. Bühler
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1506)

Abstract

Encapsulated hepatocyte transplantation and encapsulated mesenchymal stem cell transplantation are newly developed potential treatments for acute and chronic liver diseases, respectively. Cells are microencapsulated in biocompatible semipermeable alginate-based hydrogels. Microspheres protect cells against antibodies and immune cells, while allowing nutrients, small/medium size proteins and drugs to diffuse inside and outside the polymer matrix. Microencapsulated cells are assessed in vitro and designed for experimental transplantation and for future clinical applications.

Here, we describe the protocol for microencapsulation of hepatocytes and mesenchymal stem cells within hybrid poly(ethylene glycol)-alginate hydrogels.

Key words

Encapsulation Capsule Microspheres Microbeads Alginate Hydrogels Hepatocyte Mesenchymal stem cells Liver failure Hepatitis Fibrosis Cirrhosis 

Notes

Acknowledgments

This work was supported by Swiss National Science Foundation (SNSF) grant 205322 130572/1 and 205321 141286/1, the Insuleman Foundation, the Foundation Privée des HUG, and the Geneva University Hospital and Medical School.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Raphael P. H. Meier
    • 1
  • Elisa Montanari
    • 1
  • Philippe Morel
    • 1
  • Joël Pimenta
    • 1
  • Henk-Jan Schuurman
    • 1
  • Christine Wandrey
    • 2
  • Sandrine Gerber-Lemaire
    • 2
  • Redouan Mahou
    • 2
  • Leo H. Bühler
    • 1
  1. 1.Department of Surgery, Visceral and Transplant SurgeryGeneva University Hospital and Medical SchoolGenevaSwitzerland
  2. 2.Interfaculty Institute of Bioengineering and Institute of Chemical Sciences and EngineeringEcole Polytechnique Fédérale de Lausanne (EPFL)LausanneSwitzerland

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