Advertisement

Minimally Invasive Liver Preconditioning for Hepatocyte Transplantation in Rats

  • Martin Gaillard
  • Ibrahim Dagher
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1506)

Abstract

In the context of cell transplantation in the liver parenchyma, preconditioning is essential to enhance cell engraftment and liver repopulation. The authors have developed a minimally invasive technique of temporary portal embolization using an absorbable material, called reversible portal vein embolization. We hereby describe the method for isolating hepatocytes from a donor rat before transplanting hepatocytes after reversible portal vein embolization in the recipient.

Key words

Hepatocyte transplantation Liver preconditioning Liver perfusion Hepatocyte isolation Portal vein embolization 

References

  1. 1.
    Grompe M (2006) Principles of therapeutic liver repopulation. J Inherit Metab Dis 29(2–3):421–425. doi: 10.1007/s10545-006-0311-2 CrossRefPubMedGoogle Scholar
  2. 2.
    Gupta S, Rajvanshi P, Sokhi R, Slehria S, Yam A, Kerr A, Novikoff PM (1999) Entry and integration of transplanted hepatocytes in rat liver plates occur by disruption of hepatic sinusoidal endothelium. Hepatology 29(2):509–519. doi: 10.1002/hep.510290213 CrossRefPubMedGoogle Scholar
  3. 3.
    Koenig S, Krause P, Schmidt TK, Rave-Fraenk M, Rothe H, Hermann RM, Becker H, Hess CF, Christiansen H (2008) Irradiation as preparative regimen for hepatocyte transplantation causes prolonged cell cycle block. Int J Radiat Biol 84(4):285–298. doi: 10.1080/09553000801953359 CrossRefPubMedGoogle Scholar
  4. 4.
    Joseph B, Kumaran V, Berishvili E, Bhargava KK, Palestro CJ, Gupta S (2006) Monocrotaline promotes transplanted cell engraftment and advances liver repopulation in rats via liver conditioning. Hepatology 44(6):1411–1420. doi: 10.1002/hep.21416 CrossRefPubMedGoogle Scholar
  5. 5.
    Laconi E, Oren R, Mukhopadhyay DK, Hurston E, Laconi S, Pani P, Dabeva MD, Shafritz DA (1998) Long-term, near-total liver replacement by transplantation of isolated hepatocytes in rats treated with retrorsine. Am J Pathol 153(1):319–329. doi: 10.1016/S0002-9440(10)65574-5, S0002-9440(10)65574-5 [pii]CrossRefPubMedPubMedCentralGoogle Scholar
  6. 6.
    Ilan Y, Roy-Chowdhury N, Prakash R, Jona V, Attavar P, Guha C, Tada K, Roy-Chowdhury J (1997) Massive repopulation of rat liver by transplantation of hepatocytes into specific lobes of the liver and ligation of portal vein branches to other lobes. Transplantation 64(1):8–13CrossRefPubMedGoogle Scholar
  7. 7.
    Guha C, Parashar B, Deb NJ, Garg M, Gorla GR, Singh A, Roy-Chowdhury N, Vikram B, Roy-Chowdhury J (2002) Normal hepatocytes correct serum bilirubin after repopulation of Gunn rat liver subjected to irradiation/partial resection. Hepatology 36(2):354–362. doi: 10.1053/jhep.2002.34516, S0270913902000393 [pii]CrossRefPubMedGoogle Scholar
  8. 8.
    Dagher I, Boudechiche L, Branger J, Coulomb-Lhermine A, Parouchev A, Sentilhes L, Lin T, Groyer-Picard MT, Vons C, Hadchouel M, Pariente D, Andreoletti M, Franco D, Weber A (2006) Efficient hepatocyte engraftment in a nonhuman primate model after partial portal vein embolization. Transplantation 82(8):1067–1073. doi: 10.1097/01.tp.0000236103.99456.8f, 00007890-200610270-00011 [pii]CrossRefPubMedGoogle Scholar
  9. 9.
    de Baere T, Roche A, Elias D, Lasser P, Lagrange C, Bousson V (1996) Preoperative portal vein embolization for extension of hepatectomy indications. Hepatology 24(6):1386–1391CrossRefPubMedGoogle Scholar
  10. 10.
    Dagher I, Nguyen TH, Groyer-Picard MT, Lainas P, Mainot S, Guettier C, Pariente D, Franco D, Weber A (2009) Efficient hepatocyte engraftment and long-term transgene expression after reversible portal embolization in nonhuman primates. Hepatology 49(3):950–959. doi: 10.1002/hep.22739 CrossRefPubMedGoogle Scholar
  11. 11.
    Lainas P, Boudechiche L, Osorio A, Coulomb A, Weber A, Pariente D, Franco D, Dagher I (2008) Liver regeneration and recanalization time course following reversible portal vein embolization. J Hepatol 49(3):354–362. doi: 10.1016/j.jhep.2008.01.034, S0168-8278(08)00136-0 [pii]CrossRefPubMedGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.INSERM U1193, Paul Brousse HospitalVillejuifFrance
  2. 2.Department of Digestive Minimally Invasive SurgeryAntoine Béclère HospitalClamartFrance
  3. 3.Faculté de Médecine Paris-SudParis-Saclay UniversityOrsayFrance

Personalised recommendations