Cross-Linked Enzyme Aggregates for Applications in Aqueous and Nonaqueous Media
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Extensive cross-linking of a precipitate of a protein by a cross-linking reagent (glutaraldehyde has been most commonly used) creates an insoluble enzyme preparation called cross-linked enzyme aggregates (CLEAs). CLEAs show high stability and performance in conventional aqueous as well as nonaqueous media. These are also stable at fairly high temperatures. CLEAs with more than one kind of enzyme activity can be prepared, and such CLEAs are called combi-CLEAs or multipurpose CLEAs. Extent of cross-linking often influences their morphology, stability, activity, and enantioselectivity.
KeywordsCross-linking agents Glutaraldehyde Cross-linked enzyme aggregates Immobilization Thermal stability Enantioselectivity
This work was supported by funds obtained from Department of Science and Technology [Grant No.: SR/SO/BB-68/2010] and Department of Biotechnology [Grant No.: BT/PR14103/BRB/10/808/2010], both Government of India organizations. Finally, we thank past members of our research group; Dr. Kalyani Mondal, Dr. Shweta Shah, Dr. Abir Majumder, Dr. Sohel Dalal, and Veena Singh, whose work has been described/quoted in this chapter.
- 8.Sheldon RA, Schoevaart R, van Langen LM (2006) Cross-linked enzyme aggregates. In: Guisan JM (ed) Immobilization of enzymes and cells. Humana Press, Totowa, NJ, p 43Google Scholar
- 13.Sheldon RA (2006) Immobilization of enzymes as cross-linked enzyme aggregates: a simple method for improving performance. In: Patel RN (ed) Biocatalysis in the pharmaceutical and biotechnology industries. CRC Press, Boca Raton, NY, pp 350–362Google Scholar
- 26.Ribero MH, Rabaca M (2011) Cross-linked enzyme aggregates of naringinase: novel biocatalysts for naringin hydrolysis. Enzym Res 2011:851272Google Scholar
- 37.Mukherjee J, Gupta MN (2015) Paradigm shifts in our view on inclusion bodies. Curr Biochem Eng 2:1–9Google Scholar