Assaying Germination and Seedling Responses of Arabidopsis to Karrikins

  • Mark T. Waters
  • Gavin R. Flematti
  • Steven M. Smith
Part of the Methods in Molecular Biology book series (MIMB, volume 1497)


Karrikins are a small family of naturally occurring plant growth regulators present in the smoke and char produced from burning plant material in wildfires. They can stimulate germination of dormant seed and can influence seedling morphogenesis. Although Arabidopsis thaliana is not considered to be a smoke-responsive species, karrikins will stimulate seed germination under the appropriate circumstances and will cause repression of hypocotyl elongation in low light. This chapter describes how to conduct assays of the activity of karrikins on Arabidopsis seeds and seedlings. The methods presented can potentially be modified for use in a range of Arabidopsis genotypes or in other plant species.

Key words

Karrikins Arabidopsis thaliana Seed germination Dormancy Hypocotyl elongation Photomorphogenesis 


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Mark T. Waters
    • 1
    • 2
  • Gavin R. Flematti
    • 2
  • Steven M. Smith
    • 3
    • 4
  1. 1.Australian Research Council Centre of Excellence in Plant Energy BiologyUniversity of Western AustraliaCrawleyAustralia
  2. 2.School of Chemistry and BiochemistryUniversity of Western AustraliaCrawleyAustralia
  3. 3.School of Biological SciencesUniversity of TasmaniaHobartAustralia
  4. 4.State Key Laboratory of Plant Genomics & National Center for Plant Gene Research (Beijing), Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijingChina

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