Imaging Golgi Outposts in Fixed and Living Neurons
Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.
Key wordsNeurons Cultures Dendrites Golgi outposts Confocal microscopy Live imaging
Research at the Laboratory of Neurobiology at INIMEC-CONICET-UNC is supported by grants from FONCyT, CONICET to A.C. M.B. is supported by a return home grant from ISN and FONCyT grant (PICT D 2013-1525). A.C. and M.B. are staff scientists from CONICET.
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