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Imaging Golgi Outposts in Fixed and Living Neurons

  • Mariano Bisbal
  • Gonzalo Quassollo
  • Alfredo Caceres
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1496)

Abstract

Here we describe the use of confocal microscopy in combination with antibodies specific to Golgi proteins to visualize dendritic Golgi outposts (GOPs) in cultured hippocampal pyramidal neurons. We also describe the use of spinning disk confocal microscopy, in combination with ectopically expressed glycosyltransferases fused to GFP variants, to visualize GOPs in living neurons.

Key words

Neurons Cultures Dendrites Golgi outposts Confocal microscopy Live imaging 

Notes

Acknowledgements

Research at the Laboratory of Neurobiology at INIMEC-CONICET-UNC is supported by grants from FONCyT, CONICET to A.C. M.B. is supported by a return home grant from ISN and FONCyT grant (PICT D 2013-1525). A.C. and M.B. are staff scientists from CONICET.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Mariano Bisbal
    • 1
    • 2
    • 3
  • Gonzalo Quassollo
    • 1
    • 2
    • 3
  • Alfredo Caceres
    • 1
    • 2
    • 3
  1. 1.Laboratorio NeurobiologíaInstituto Investigación Médica Mercedes y Martín Ferreyra (INIMEC-CONICET)CórdobaArgentina
  2. 2.Universidad Nacional de Córdoba (Argentina)CórdobaArgentina
  3. 3.Instituto Universitario Ciencias Biomédicas CórdobaCórdobaArgentina

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