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Eliciting Epitope-Specific CD8+ T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols

Part of the Methods in Molecular Biology book series (MIMB,volume 1494)

Abstract

CD8+ cytotoxic T lymphocytes confer protection against infectious diseases caused by viruses, bacteria, and parasites. Hence, significant efforts have been invested into devising ways to generate CD8+ T cell-targeted vaccines. Generation of microbe-free protein subunit vaccines requires a thorough knowledge of protective target antigens. Such antigens are proteolytically processed peptides presented by MHC class I molecules. To induce a robust antigen-specific CD8+ T cell response through vaccination, it is essential to formulate the antigen with an effective adjuvant. Here, we describe a versatile method for generating high-frequency antigen-specific CD8+ T cells through immunization of mice using the invariant natural killer T cell agonist α-galactosylceramide as the adjuvant.

Key words

  • Adjuvant
  • α-Galactosylceramide
  • Antigen-specific CD8+ T cells
  • Microbial protein antigens
  • Mouse immunization

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Acknowledgements

Supported by Vanderbilt University Discovery Grant as well as VA Merit Award (BX001444) and NIH Contracts (AI040079), Research (AI042284, HL121139), Core (CA068485, DK058404), and Center (CA068485) grants.

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Gilchuk, P., Knight, F.C., Wilson, J.T., Joyce, S. (2017). Eliciting Epitope-Specific CD8+ T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols. In: Fox, C. (eds) Vaccine Adjuvants. Methods in Molecular Biology, vol 1494. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6445-1_25

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