Eliciting Epitope-Specific CD8+ T Cell Response by Immunization with Microbial Protein Antigens Formulated with α-Galactosylceramide: Theory, Practice, and Protocols

  • Pavlo Gilchuk
  • Frances C. Knight
  • John T. Wilson
  • Sebastian JoyceEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1494)


CD8+ cytotoxic T lymphocytes confer protection against infectious diseases caused by viruses, bacteria, and parasites. Hence, significant efforts have been invested into devising ways to generate CD8+ T cell-targeted vaccines. Generation of microbe-free protein subunit vaccines requires a thorough knowledge of protective target antigens. Such antigens are proteolytically processed peptides presented by MHC class I molecules. To induce a robust antigen-specific CD8+ T cell response through vaccination, it is essential to formulate the antigen with an effective adjuvant. Here, we describe a versatile method for generating high-frequency antigen-specific CD8+ T cells through immunization of mice using the invariant natural killer T cell agonist α-galactosylceramide as the adjuvant.

Key words

Adjuvant α-Galactosylceramide Antigen-specific CD8+ T cells Microbial protein antigens Mouse immunization 



Supported by Vanderbilt University Discovery Grant as well as VA Merit Award (BX001444) and NIH Contracts (AI040079), Research (AI042284, HL121139), Core (CA068485, DK058404), and Center (CA068485) grants.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Pavlo Gilchuk
    • 1
    • 2
  • Frances C. Knight
    • 3
  • John T. Wilson
    • 3
    • 4
  • Sebastian Joyce
    • 1
    • 2
    Email author
  1. 1.Veterans Administration Tennessee Valley Healthcare SystemUS Department of Veterans AffairsNashvilleUSA
  2. 2.Department of Pathology, Microbiology and Immunology, School of MedicineVanderbilt UniversityNashvilleUSA
  3. 3.Department of Biomedical Engineering, School of EngineeringVanderbilt UniversityNashvilleUSA
  4. 4.Department of Chemical & Biomolecular Engineering, School of EngineeringVanderbilt UniversityNashvilleUSA

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