Genotyping pp 197-202 | Cite as

Efficient, Cost-Effective, High-Throughput, Multilocus Sequencing Typing (MLST) Method, NGMLST, and the Analytical Software Program MLSTEZ

Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1492)

Abstract

Multilocus sequence typing (MLST) has become the preferred method for genotyping many biological species. It can be used to identify major phylogenetic clades, molecular groups, or subpopulations of a species, as well as individual strains or clones. However, conventional MLST is costly and time consuming, which limits its power for genotyping large numbers of samples. Here, we describe a new MLST method that uses next-generation sequencing, a multiplexing protocol, and appropriate analytical software to provide accurate, rapid, and economical MLST genotyping of 96 or more isolates in a single assay.

Key words

Multilocus sequence typing Genotyping Next-generation sequencing Multiplex PCR 

Notes

Acknowledgment

This work was supported by Public Health Service Grants AI73896 and AI93257 (JRP).

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Division of Infectious Diseases, Department of MedicineDuke University Medical CenterDurhamUSA

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