Comparative Activity-Based Flavin-Dependent Oxidase Profiling
Activity-based protein profiling (ABPP) has become a powerful chemoproteomic technology allowing for the dissection of complex ligand–protein interactions in their native cellular environment. One of the biggest challenges for ABPP is the extension of the proteome coverage. In this chapter a new ABPP strategy dedicated to monoamine oxidases (MAO) is presented. These enzymes are representative examples of flavin-dependent oxidases, playing a crucial role in the regulation of nervous system signaling.
Key wordsActivity-based protein profiling Click chemistry Flavoproteins Mitsunobu reaction Monoamine oxidase Oxidoreductase Pargyline Target identification
Activity-based protein profiling
Flavin adenine dinucleotide
Fetal calf serum
Gas chromatography mass spectrometry
High-resolution mass spectrometry
Sodium dodecyl sulfate–polyacrylamide gel electrophoresis
The research by the authors was funded by the Austrian Science Fund (FWF) grant W901-B05 DK Molecular Enzymology and profited tremendously from the collaboration with Prof. S. A. Sieber (TU Munich, Germany), Prof. D. E. Edmondson (Emory University, Atlanta, USA) and Prof. Dr. W. Berger (Medical University of Vienna, Vienna).
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