Cleavable Linkers in Chemical Proteomics Applications

  • Yinliang Yang
  • Marko Fonović
  • Steven H. L. VerhelstEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1491)


The discovery of the protein targets of small molecule probes is a crucial aspect of activity-based protein profiling and chemical biology. Mass spectrometry is the primary method for target identification, and in the last decade, cleavable linkers have become a popular strategy to facilitate protein enrichment and identification. In this chapter, we provide an overview of cleavable linkers used in chemical proteomics approaches, discuss their different chemistries, and describe how they aid in protein identification.

Key words

Activity-based probes Chemical proteomics Cleavable linkers Click chemistry Enrichment Mass spectrometry Protein identification Target discovery 



We acknowledge funding by the Chinese Scholarship Council (to Y.Y.), the Slovenian Research Agency (ARRS grant to M.F.), the Deutsche Forschungsgemeinschaft (to S.V.) and the Ministerium für Innovation, Wissenschaft und Forschung des Landes Nordrhein- Westfalen.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Yinliang Yang
    • 1
  • Marko Fonović
    • 2
  • Steven H. L. Verhelst
    • 3
    • 4
    Email author
  1. 1.Lehrstuhl für Chemie der BiopolymereTechnische Universität MünchenFreisingGermany
  2. 2.Department of Biochemistry, Molecular and Structural BiologyJožef Stefan InstituteLjubljanaSlovenia
  3. 3.Department of Cellular and Molecular MedicineKU Leuven - University of LeuvenLeuvenBelgium
  4. 4.Leibniz Institute for AnalyticalSciences ISASDortmundGermany

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