ERK Signaling pp 317-335 | Cite as

Discovering Functional ERK Substrates Regulating Caenorhabditis elegans Germline Development

  • Jessica Jie Chen
  • Swathi ArurEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1487)


The Rat Sarcoma (RAS) GTPAse-mediated extracellular signal-regulated kinase (ERK) pathway regulates multiple biological processes across metazoans. In particular during Caenorhabditis elegans oogenesis, ERK signaling has been shown to regulate over seven distinct biological processes in a temporal and sequential manner. To fully elucidate how ERK signaling cascade orchestrates these different biological processes in vivo, identification of the direct functional substrates of the pathway is critical. This chapter describes the methods that were used to identify ERK substrates in a global manner and study their functions in the germline. These approaches can also be generally applied to study ERK-dependent biological processes in other systems.

Key words

C. elegans oogenesis ERK substrates Functional genomics 



J.J.C. is funded by the UT Graduate School of Biomedical Sciences Dean’s Scholarship. NIH RO1 GM 98200, American Cancer Society Grant ACS RSG014-044-DDC and CPRIT RP160023 grants fund work in the Arur Lab.


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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.The University of Texas Graduate School of Biomedical SciencesHoustonUSA
  2. 2.Department of GeneticsThe University of Texas MD Anderson Cancer CenterHoustonUSA

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