Understanding RNA-Chromatin Interactions Using Chromatin Isolation by RNA Purification (ChIRP)
ChIRP is a novel and easy-to-use technique for studying long noncoding RNA (lncRNA)-chromatin interactions. RNA and chromatin are cross-linked in vivo using formaldehyde or glutaraldehyde, and purified using biotinylated antisense oligonucleotides that hybridize to the target RNA. Co-precipitated DNA is then purified and analyzed by quantitative PCR (qPCR) or high-throughput sequencing.
Key wordslncRNA Chromatin High-throughput sequencing Cross-linking
This work was supported by the Singapore Agency for Science, Technology, and Research (A*STAR to C.C.) and the US National Institutes of Healthy (H.Y.C.). H.Y.C. is an Early Career Scientist of the Howard Hughes Medical Institute.
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