Drosophila pp 291-302 | Cite as

Protocols to Study Aging in Drosophila

  • Matthew D. W. Piper
  • Linda PartridgeEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1478)


The fruit fly Drosophila melanogaster offers a host of advantages for studying the biology of aging: a well-understood biology, a wide range of genetic reagents, well-defined dietary requirements, and a relatively short life span, with a median of ~80 days and maximum ~100 days. Several phenotypes can be used to assess the aging process, but the simplest and most widely used metric is length of life. Here we describe a standard life span assay for Drosophila housed on a simple sugar/yeast diet.

Key words

Drosophila melanogaster Life span Aging Method Diet Genetic interventions Pharmacological interventions Backcross 



We would like to thank members of the Partridge and Piper laboratories who contributed helpful suggestions and tips. In particular: S. Grönke, T. Niccoli, A. Tillmann, and N. Woodling. We acknowledge the following sources of funding: the Royal Society (UF100158 & RG110303), AustralianResearch Council (FT150100237) (MDWP); the Wellcome Trust UK (098565/Z/12/Z), Max Planck Society, and the European Research Council under the European Unions Seventh Framework Programme (FP7/2007-2013), European Research Council grant agreement 268739 (L.P.)


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Healthy Ageing and Department Genetics, Evolution and EnvironmentUniversity College LondonLondonUK
  2. 2.Max Planck Institute for Biology of AgeingKölnGermany
  3. 3.School of Biological SciencesMonash UniversityClaytonAustralia

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