Drosophila pp 263-277

Part of the Methods in Molecular Biology book series (MIMB, volume 1478) | Cite as

Chromatin Immunoprecipitation for Analyzing Transcription Factor Binding and Histone Modifications in Drosophila

  • Yad Ghavi-Helm
  • Bingqing Zhao
  • Eileen E. M. Furlong
Protocol

Abstract

Chromatin immunoprecipitation followed by next-generation sequencing (ChIP-seq) is an invaluable technique to assess transcription factor binding and histone modifications in a genome-wide manner, an essential step towards understanding the mechanisms that govern embryonic development. Here, we provide a detailed protocol for all steps involved in generating a ChIP-seq library, starting from embryo collection, fixation, chromatin preparation, immunoprecipitation, and finally library preparation. The protocol is optimized for Drosophila embryos, but can be easily adapted for any model organism. The resulting library is suitable for sequencing on an Illumina HiSeq or MiSeq platform.

Key words

Drosophila Transcription Development Chromatin immunoprecipitation ChIP-seq Next-generation sequencing 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Yad Ghavi-Helm
    • 1
  • Bingqing Zhao
    • 1
  • Eileen E. M. Furlong
    • 1
  1. 1.European Molecular Biology LaboratoryGenome Biology UnitHeidelbergGermany

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