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Analytical Methods for Secondary Metabolite Detection

  • Judith Taibon
  • Hermann StrasserEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1477)

Abstract

The entomopathogenic fungi Metarhizium brunneum, Beauveria bassiana, and B. brongniartii are widely applied as biological pest control agent in OECD countries. Consequently, their use has to be flanked by a risk management approach, which includes the need to monitor the fate of their relevant toxic metabolites. There are still data gaps claimed by regulatory authorities pending on their identification and quantification of relevant toxins or secondary metabolites. In this chapter, analytical methods are presented allowing the qualitative and quantitative analysis of the relevant toxic B. brongniartii metabolite oosporein and the three M. brunneum relevant destruxin (dtx) derivatives dtx A, dtx B, and dtx E.

Key words

Secondary metabolite Toxin Oosporein Destruxin Persistence Risk assessment Analyte monitoring Analytical tool HPLC–DAD HPLC–DAD–QTOF–MS/MS 

Notes

Acknowledgements

This research has been supported by the European Community’s Seventh Framework Programme grant (FP7_ENV.2011.3.1.9-1 ECO-INNOVATION, INBIOSOIL, Grant Agreement No. 282767). We grateful acknowledge the contribution from PD Dr. Christoph Seger, Dr. Sonja Sturm and Prof. Dr. Hermann Stuppner (all University Innsbruck) who provided their expertise within this project.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Pharmacy, Department of Pharmacognosy, CCB – Centrum of Chemistry and BiomedicineUniversity of InnsbruckInnsbruckAustria
  2. 2.Institute of MicrobiologyUniversity of InnsbruckInnsbruckAustria

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