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Methods for Microencapsulated Porcine Islet Production

  • Masayuki Shimoda
  • Shinichi Matsumoto
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1479)

Abstract

Allogeneic islet transplantation has become a viable option for the treatment of unstable type 1 diabetes; however, donor shortage and the necessity for immunosuppressive drugs are two major drawbacks of this treatment. Microencapsulated porcine islets can solve both of these drawbacks, and clinical trials have been conducted. Previous clinical trials demonstrated the safety and marginal efficacy of this treatment; therefore, it is necessary to improve its efficacy. The production of microencapsulated porcine islets consists of pancreas procurement, islet isolation, and microencapsulation. In this chapter, we describe our current methods for porcine pancreas procurement, islet isolation, and microencapsulation.

Key words

Pancreas procurement Porcine islets Microencapsulation Islet transplantation Donor shortage Type 1 diabetes 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.National Center for Global Health and MedicineTokyoJapan
  2. 2.Otsuka Pharmaceutical Factory Inc.NarutoJapan

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