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High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells

  • Sampada A. Shahane
  • Kana Nishihara
  • Menghang XiaEmail author
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1473)

Abstract

Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.

Key words

Micronuclei High-content imaging Genotoxicity 
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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sampada A. Shahane
    • 1
  • Kana Nishihara
    • 1
  • Menghang Xia
    • 1
    Email author
  1. 1.National Center for Advancing Translational SciencesNational Institutes of HealthBethesdaUSA

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