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High-Throughput and High-Content Micronucleus Assay in CHO-K1 Cells

  • Sampada A. Shahane
  • Kana Nishihara
  • Menghang XiaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1473)

Abstract

Visualization of micronuclei induction by chemicals and drugs enables measurement of possible compound genotoxicity. A loss of entire chromosome or a fragment of chromosome can lead to formation of micronuclei (MNi). The in vitro micronucleus assay can be conducted using nuclear dyes with high-content imaging platforms. This chapter describes the cytochalasin block method of measuring micronuclei in CHO-K1 cell lines.

Key words

Micronuclei High-content imaging Genotoxicity 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Sampada A. Shahane
    • 1
  • Kana Nishihara
    • 1
  • Menghang Xia
    • 1
    Email author
  1. 1.National Center for Advancing Translational SciencesNational Institutes of HealthBethesdaUSA

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