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Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway

  • Jinghua Zhao
  • Sunita J. Shukla
  • Menghang XiaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1473)

Abstract

The antioxidant response element (ARE) signaling pathway plays an important role in the amelioration of cellular oxidative stress. Thus, assays that detect this pathway can be useful for identifying chemicals that induce or inhibit oxidative stress signaling. The focus of this chapter is to describe a cell-based ARE assay in a quantitative high-throughput screening (qHTS) format to test a large collection of compounds that induce nuclear factor erythroid 2-related factor (Nrf2)/ARE signaling. The assay is described through cell handling, assay preparation, and instrument usage.

Key words

Antioxidant response element (ARE) Reactive oxygen species (ROS) Nuclear factor erythroid 2-related factor (Nrf2) Quantitative high-throughput screening (qHTS) β-lactamase (bla) Fluorescence Resonance Energy Transfer (FRET) 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.National Center for Advancing Translational SciencesNational Institutes of HealthBethesdaUSA
  2. 2.Center for Drug Evaluation and ResearchU.S. Food and Drug AdministrationSilver SpringUSA

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