Cell-Based Assay for Identifying the Modulators of Antioxidant Response Element Signaling Pathway
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The antioxidant response element (ARE) signaling pathway plays an important role in the amelioration of cellular oxidative stress. Thus, assays that detect this pathway can be useful for identifying chemicals that induce or inhibit oxidative stress signaling. The focus of this chapter is to describe a cell-based ARE assay in a quantitative high-throughput screening (qHTS) format to test a large collection of compounds that induce nuclear factor erythroid 2-related factor (Nrf2)/ARE signaling. The assay is described through cell handling, assay preparation, and instrument usage.
Key wordsAntioxidant response element (ARE) Reactive oxygen species (ROS) Nuclear factor erythroid 2-related factor (Nrf2) Quantitative high-throughput screening (qHTS) β-lactamase (bla) Fluorescence Resonance Energy Transfer (FRET)
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