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Mitochondrial Membrane Potential Assay

  • Srilatha Sakamuru
  • Matias S. Attene-Ramos
  • Menghang XiaEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1473)

Abstract

Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells. A homogenous cell-based MMP assay was optimized and performed in a 1536-well plate format to screen several compound libraries for mitochondrial toxicity by evaluating the effects of chemical compounds on MMP.

Key words

Mitochondrial membrane potential (MMP) Mitochondrial membrane potential indicator (m-MPI) Mitochondrial toxicity 1536-well plate format Mesoxalonitrile 4-trifluoromethoxyphenylhydrazone (FCCP) 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Srilatha Sakamuru
    • 1
  • Matias S. Attene-Ramos
    • 1
  • Menghang Xia
    • 1
    Email author
  1. 1.National Center for Advancing Translational Sciences, National Institutes of HealthBethesdaUSA

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