PaperClip: A Simple Method for Flexible Multi-Part DNA Assembly
Joining DNA sequences to create linear and circular constructs is a basic requirement in molecular biology. Here we describe PaperClip, a recently developed method, which enables assembly of multiple DNA sequences in one reaction in a combinatorial manner. In contrast to other homology-based multi-part assembly methods currently available, PaperClip allows assembly of a given set of parts in any order without requiring specific single-use oligonucleotides for each assembly order.
Key wordsMolecular cloning DNA assembly Single-pot assembly Oligonucleotide annealing Ligation PAGE PCR Agarose gel electrophoresis CPEC SLiCE Cell extract Selection
This work was supported by Engineering and Physical Sciences Research Council [EP/J02175x/1].