SpeedyGenes: Exploiting an Improved Gene Synthesis Method for the Efficient Production of Synthetic Protein Libraries for Directed Evolution

  • Andrew Currin
  • Neil Swainston
  • Philip J. Day
  • Douglas B. Kell
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1472)

Abstract

Gene synthesis is a fundamental technology underpinning much research in the life sciences. In particular, synthetic biology and biotechnology utilize gene synthesis to assemble any desired DNA sequence, which can then be incorporated into novel parts and pathways. Here, we describe SpeedyGenes, a gene synthesis method that can assemble DNA sequences with greater fidelity (fewer errors) than existing methods, but that can also be used to encode extensive, statistically designed sequence variation at any position in the sequence to create diverse (but accurate) variant libraries. We summarize the integrated use of GeneGenie to design DNA and oligonucleotide sequences, followed by the procedure for assembling these accurately and efficiently using SpeedyGenes.

Key words

Directed evolution Error correction Gene synthesis Protein libraries Synthetic biology 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Andrew Currin
    • 1
    • 2
    • 3
  • Neil Swainston
    • 1
    • 3
    • 4
  • Philip J. Day
    • 1
    • 3
    • 5
  • Douglas B. Kell
    • 1
    • 2
    • 3
  1. 1.Manchester Institute of BiotechnologyThe University of ManchesterManchesterUK
  2. 2.School of ChemistryThe University of ManchesterManchesterUK
  3. 3.Centre for Synthetic Biology of Fine and Speciality Chemicals (SYNBIOCHEM)The University of ManchesterManchesterUK
  4. 4.School of Computer ScienceThe University of ManchesterManchesterUK
  5. 5.Faculty of Medical and Human SciencesThe University of ManchesterManchesterUK

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