Synthetic DNA pp 217-235

Part of the Methods in Molecular Biology book series (MIMB, volume 1472)

Immobilized MutS-Mediated Error Removal of Microchip-Synthesized DNA

Protocol

Abstract

Applications of microchip-synthesized oligonucleotides for de novo gene synthesis are limited primarily by their high error rates. The mismatch binding protein MutS, which can specifically recognize and bind to mismatches, is one of the cheapest tools for error correction of synthetic DNA. Here, we describe a protocol for removing errors in microchip-synthesized oligonucleotides and for the assembly of DNA segments using these oligonucleotides. This protocol can also be used in traditional de novo gene DNA synthesis.

Key words

MutS Microchip-synthesized oligonucleotides Gene assemble Error removal De novo gene synthesis 

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  • Wen Wan
    • 1
  • Dongmei Wang
    • 1
  • Xiaolian Gao
    • 1
    • 2
  • Jiong Hong
    • 1
    • 2
  1. 1.School of Life ScienceUniversity of Science and Technology of ChinaHefeiPeople’s Republic of China
  2. 2.Hefei National Laboratory for Physical Science at the MicroscaleHefei, 230026People’s Republic of China

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