Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)
The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.
Key wordsSynthetic biology DNA assembly High throughput Yeast homologous recombination DNA synthesis
This work was funded by Defense Advanced Research Projects Agency (DARPA) Living Foundries grant HR001-12-3-0006.
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