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Synthetic DNA pp 187-192 | Cite as

Efficient Assembly of DNA Using Yeast Homologous Recombination (YHR)

  • Sunil ChandranEmail author
  • Elaine Shapland
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1472)

Abstract

The assembly of multiple DNA parts into a larger DNA construct is a requirement in most synthetic biology laboratories. Here we describe a method for the efficient, high-throughput, assembly of DNA utilizing the yeast homologous recombination (YHR). The YHR method utilizes overlapping DNA parts that are assembled together by Saccharomyces cerevisiae via homologous recombination between designed overlapping regions. Using this method, we have successfully assembled up to 12 DNA parts in a single reaction.

Key words

Synthetic biology DNA assembly High throughput Yeast homologous recombination DNA synthesis 

Notes

Acknowledgements

This work was funded by Defense Advanced Research Projects Agency (DARPA) Living Foundries grant HR001-12-3-0006.

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Copyright information

© Springer Science+Business Media New York 2017

Authors and Affiliations

  1. 1.Amyris, Inc.EmeryvilleUSA

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