Abstract
The visualization of meiotic chromosomes and their associated protein structures in both wild-type and mutant cells adds valuable insight into the molecular pathways that underlie reproductive cell formation. Here we describe basic methodology for visualizing meiotic chromosomes in a long-standing model organism for investigating the molecular and cell biology of meiosis, the budding yeast, S. cerevisiae. This chapter furthermore highlights a variety of conditional expression regimes that can be used to understand the dynamics and/or developmental constraints of chromosomal protein structures; such dynamic aspects of the macromolecular structures that mediate meiotic chromosome biology are typically not obvious from standard protein visualization experiments.
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MacQueen, A.J., Rockmill, B. (2017). Analysis of Meiotic Chromosome-Associated Protein Dynamics Using Conditional Expression in Budding Yeast. In: Stuart, D. (eds) Meiosis. Methods in Molecular Biology, vol 1471. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-6340-9_8
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DOI: https://doi.org/10.1007/978-1-4939-6340-9_8
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Publisher Name: Humana Press, New York, NY
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Online ISBN: 978-1-4939-6340-9
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