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High-Throughput, Liquid-Based Genome-Wide RNAi Screening in C. elegans

  • Linda P. O’Reilly
  • Ryan R. Knoerdel
  • Gary A. Silverman
  • Stephen C. PakEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1470)

Abstract

RNA interference (RNAi) is a process in which double-stranded RNA (dsRNA) molecules mediate the inhibition of gene expression. RNAi in C. elegans can be achieved by simply feeding animals with bacteria expressing dsRNA against the gene of interest. This “feeding” method has made it possible to conduct genome-wide RNAi experiments for the systematic knockdown and subsequent investigation of almost every single gene in the genome. Historically, these genome-scale RNAi screens have been labor and time intensive. However, recent advances in automated, high-throughput methodologies have allowed the development of more rapid and efficient screening protocols. In this report, we describe a fast and efficient, liquid-based method for genome-wide RNAi screening.

Key words

RNAi Genome wide High-throughput screening C. elegans Arrayscan 

Notes

Acknowledgment

This work was supported by a grant from the National Institutes of Health (DK096990) to GAS.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Linda P. O’Reilly
    • 1
    • 2
  • Ryan R. Knoerdel
    • 1
    • 2
  • Gary A. Silverman
    • 1
    • 2
    • 3
  • Stephen C. Pak
    • 1
    • 2
    • 3
    Email author
  1. 1.Departments of PediatricsUniversity of Pittsburgh School of MedicinePittsburghUSA
  2. 2.Division of Newborn MedicineChildren’s Hospital of Pittsburgh of UPMCPittsburghUSA
  3. 3.Departments of PediatricsWashington University School of MedicineSt LouisUSA

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