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Chromatin Immunoprecipitation for Detecting Epigenetic Marks on Plant Nucleosomes

  • Kiyotaka NagakiEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1469)

Abstract

Due to high resolution and reproducibility, chromatin immunoprecipitation (ChIP) has been used as a standard tool to investigate epigenetic marks including modified histones and specific histone variants (e.g., centromere-specific histone H3, CENH3) in this decade. Here, I describe a sensitive and low-background ChIP protocol for a wide range of plant species.

Key words

Chromatin immunoprecipitation (ChIP) Epigenetic marks Histone modification Histone variants CENH3 

References

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute of Plant Science and ResourcesOkayama UniversityKurashikiJapan

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