Exosomes in Tumor Angiogenesis

  • Karma Z. Salem
  • Michele Moschetta
  • Antonio Sacco
  • Luisa Imberti
  • Giuseppe Rossi
  • Irene M. Ghobrial
  • Salomon Manier
  • Aldo M. RoccaroEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1464)


Exosomes are small vesicles ranging in size between 30 and 150 nm, derived from the luminal membranes of the endosome and are constitutively released by fusion with the cell membrane. Several studies have revealed that exosomes play crucial roles in mediating local and systemic cell communication through the horizontal transfer of information in the form of nucleic material and proteins. This is particularly relevant in the context of the tumor-microenvironment cross talk. Here we describe the method of isolating exosomes and their role in modifying the tumor environment and more specifically in enabling metastasis and promoting angiogenesis.

Key words

Exosome Angiogenesis Cancer Tumor Microenvironment Pre-metastatic niche 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Karma Z. Salem
    • 1
  • Michele Moschetta
    • 1
  • Antonio Sacco
    • 1
  • Luisa Imberti
    • 2
  • Giuseppe Rossi
    • 2
  • Irene M. Ghobrial
    • 1
  • Salomon Manier
    • 1
  • Aldo M. Roccaro
    • 1
    • 2
    Email author
  1. 1.Department of Medical OncologyDana-Farber Cancer Institute, Harvard Medical SchoolBostonUSA
  2. 2.ASST Spedali Civili, Department of Medical OncologyCREA LaboratoryBresciaItaly

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