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Cilia pp 215-236 | Cite as

Methods to Study Centrosomes and Cilia in Drosophila

  • Swadhin Chandra Jana
  • Susana Mendonça
  • Sascha Werner
  • Monica Bettencourt-Dias
Part of the Methods in Molecular Biology book series (MIMB, volume 1454)

Abstract

Centrioles and cilia are highly conserved eukaryotic organelles. Drosophila melanogaster is a powerful genetic and cell biology model organism, extensively used to discover underlying mechanisms of centrosome and cilia biogenesis and function. Defects in centrosomes and cilia reduce fertility and affect different sensory functions, such as proprioception, olfaction, and hearing. The fly possesses a large diversity of ciliary structures and assembly modes, such as motile, immotile, and intraflagellar transport (IFT)-independent or IFT-dependent assembly. Moreover, all the diverse ciliated cells harbor centrioles at the base of the cilia, called basal bodies, making the fly an attractive model to better understand the biology of this organelle. This chapter describes protocols to visualize centrosomes and cilia by fluorescence and electron microscopy.

Key words

Drosophila Centrosome Basal body Cilia Intraflagellar transport Sensory neuron Sperm 

Notes

Acknowledgement

We apologize to colleagues whose work was not discussed or cited due to space constraints. We thank the IGC imaging unit (Light microscopy and Electron microscopy) for the help with image acquisition, and the IGC fly facility, and MBD Lab for discussions. S.C.J. and S.W. are supported by the FCT (Fundação Portuguesa para a Ciência e Tecnologia) Fellowships SFRH/BPD/87479/2012 and SFRH/BD/52176/2013, respectively. The laboratory and MBD are supported by an EMBO installation grant and an ERC starting grant.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Instituto Gulbenkian de CiênciaOeirasPortugal

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