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Sampling of Riverine or Marine Bacterial Communities in Remote Locations: From Field to Publication

  • Katja LehmannEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1452)

Abstract

This protocol describes how to sample and preserve microbial water column samples from rivers that can be used for 16S or 18S metabarcoding studies or shotgun sequencing. It further describes how to extract the DNA for sequencing and how to prepare raw Illumina MiSeq amplicon data and analyze it in the R environment.

Key words

Biodiversity Riverine microbial communities Community analysis Illumina MiSeq 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Centre for Ecology and HydrologyWallingfordUK

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