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Zebrafish pp 333-341 | Cite as

Calcium Imaging of Neuronal Activity in Free-Swimming Larval Zebrafish

  • Akira Muto
  • Koichi Kawakami
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1451)

Abstract

Visualization of neuronal activity during animal behavior is a critical step in understanding how the brain generates behavior. In the model vertebrate zebrafish, imaging of the brain has been done mostly by using immobilized fish. Here, we describe a novel method to image neuronal activity of the larval zebrafish brain during prey capture behavior. We expressed a genetically encoded fluorescent calcium indicator, GCaMP, in the optic tectum of the midbrain using the Gal4-UAS system. Tectal activity was then imaged in unrestrained larvae during prey perception. Since larval zebrafish swim only intermittently, detection of the neuronal activity is possible between swimming bouts. Our method makes functional brain imaging under natural behavioral conditions feasible and will greatly benefit the study of neuronal activities that evoke animal behaviors.

Key words

Calcium imaging GCaMP Tectum Prey capture Vision Paramecium Gal4-UAS system 

Notes

Acknowledgments

This work was supported by grants from the Ministry of Education, Culture, Sports, Science and Technology (MEXT) in Japan: KAKENHI Grant Number 24120521, 25290009, and 25650120.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Division of Molecular and Developmental Biology, Department of Genetics, National Institute of GeneticsSOKENDAI (The Graduate University for Advanced Studies)MishimaJapan

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