Correlating Whole Brain Neural Activity with Behavior in Head-Fixed Larval Zebrafish
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We present a protocol to combine behavioral recording and imaging using 2-photon laser-scanning microscopy in head-fixed larval zebrafish that express a genetically encoded calcium indicator. The steps involve restraining the larva in agarose, setting up optics that allow projection of a visual stimulus and infrared illumination to monitor behavior, and analysis of the neuronal and behavioral data.
Key wordsWhole-brain imaging Behavior 2-Photon microscopy Zebrafish
The authors would like to acknowledge the contribution of colleagues Claudia Feierstein and Florian Engert to the development of the methods described in this protocol. MBO was supported by a Marie Curie Career Integration Grant, PCIG09-GA-2011-294049. RP was supported by the Max Planck Society.
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