Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish
The zebrafish has emerged as an important model organism for understanding the cellular and molecular mechanisms of tissue regeneration. Adult zebrafish efficiently replace cardiac muscle after partial resection of their ventricle, or after transgenic ablation of cardiomyocytes. Here, we describe methodology for inducing these injuries and assaying indicators of regeneration.
Key wordsZebrafish Heart Surgery Ablation Cardiomyocyte Regeneration
This work was funded by postdoctoral fellowships from the American Heart Association to J.W. and grants from NIH (HL081674, HL131319) and American Federation for Aging Research to K.D.P. We thank Amy Dickson and Anne Knecht for reviewing and contributing to this manuscript.
- 6.Wang J, Panakova D, Kikuchi K, Holdway JE, Gemberling M, Burris JS, Singh SP, Dickson AL, Lin YF, Sabeh MK, Werdich AA, Yelon D, Macrae CA, Poss KD (2011) The regenerative capacity of zebrafish reverses cardiac failure caused by genetic cardiomyocyte depletion. Development 138:3421–3430CrossRefPubMedPubMedCentralGoogle Scholar
- 11.Kim J, Wu Q, Zhang Y, Wiens KM, Huang Y, Rubin N, Shimada H, Handin RI, Chao MY, Tuan TL, Starnes VA, Lien CL (2010) PDGF signaling is required for epicardial function and blood vessel formation in regenerating zebrafish hearts. Proc Natl Acad Sci U S A 107:17206–17210CrossRefPubMedPubMedCentralGoogle Scholar
- 18.Zhou B, Honor LB, He H, Ma Q, Oh JH, Butterfield C, Lin RZ, Melero-Martin JM, Dolmatova E, Duffy HS, Gise A, Zhou P, Hu YW, Wang G, Zhang B, Wang L, Hall JL, Moses MA, McGowan FX, Pu WT (2011) Adult mouse epicardium modulates myocardial injury by secreting paracrine factors. J Clin Invest 121:1894–1904CrossRefPubMedPubMedCentralGoogle Scholar