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Zebrafish pp 225-235 | Cite as

Methodologies for Inducing Cardiac Injury and Assaying Regeneration in Adult Zebrafish

  • Jinhu Wang
  • Kenneth D. PossEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1451)

Abstract

The zebrafish has emerged as an important model organism for understanding the cellular and molecular mechanisms of tissue regeneration. Adult zebrafish efficiently replace cardiac muscle after partial resection of their ventricle, or after transgenic ablation of cardiomyocytes. Here, we describe methodology for inducing these injuries and assaying indicators of regeneration.

Key words

Zebrafish Heart Surgery Ablation Cardiomyocyte Regeneration 

Notes

Acknowledgments

This work was funded by postdoctoral fellowships from the American Heart Association to J.W. and grants from NIH (HL081674, HL131319) and American Federation for Aging Research to K.D.P. We thank Amy Dickson and Anne Knecht for reviewing and contributing to this manuscript.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Cell BiologyDuke University Medical CenterDurhamUSA

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