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Harvesting Human Prostate Tissue Material and Culturing Primary Prostate Epithelial Cells

  • Fiona M. Frame
  • Davide Pellacani
  • Anne T. Collins
  • Norman J. Maitland
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1443)

Abstract

In order to fully explore the biology of a complex solid tumor such as prostate cancer, it is desirable to work with patient tissue. Only by working with cells from a tissue can we take into account patient variability and tumor heterogeneity. Cell lines have long been regarded as the workhorse of cancer research and it could be argued that they are of most use when considered within a panel of cell lines, thus taking into account specified mutations and variations in phenotype between different cell lines. However, often very different results are obtained when comparing cell lines to primary cells cultured from tissue. It stands to reason that cells cultured from patient tissue represents a close-to-patient model that should and does produce clinically relevant data. This chapter aims to illustrate the methods of processing, storing and culturing cells from prostate tissue, with a description of potential uses.

Key words

Prostate Tissue Primary epithelial cells Cancer Benign prostatic hyperplasia 

Notes

Acknowledgements

This work was supported by a Yorkshire Cancer Research Core Grant (Y257PA). Photo credits to: Paula Kroon, Rachel Adamson, Shona Lang, Paul Berry, Stephanie Swift.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.YCR Cancer Research Unit, Department of BiologyUniversity of YorkHeslington, North YorkshireUK

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