Analysis of Expression and Functions of Histone Deacetylase 6 (HDAC6)

  • Miao Li
  • Yan Zhuang
  • Bin ShanEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1436)


Histone deacetylase 6 (HDAC6) is a member of class IIb HDAC family. HDAC6 exists predominantly in the cytoplasm and deacetylates mainly non-histone proteins in the cytoplasm. Via its deacetylase and ubiquitin binding domains, HDAC6 regulates microtubules, cytoskeleton, intracellular trafficking, and cellular responses to stress. HDAC6 plays a central role in physiology and pathobiology in various organs and tissues. Herein we describe the methods for analysis of expression and function of HDAC6 in diverse physiological and pathological contexts.

Key words

Histone deacetylase 6 Acetylation Stress Chaperone Ubiquitin 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Department of Pathogen Biology, College of Basic Medical SciencesChina Medical UniversityShenyangPeople’s Republic of China
  2. 2.Department of MedicineTulane University School of MedicineNew OrleansUSA
  3. 3.College of Medical SciencesWashington State University at SpokaneSpokaneUSA

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