Generation of Human Liver Chimeric Mice for the Study of Human Hepatotropic Pathogens

  • Markus von Schaewen
  • Gabriela Hrebikova
  • Alexander Ploss
Protocol
First Online:
Part of the Methods in Molecular Biology book series (MIMB, volume 1438)

Abstract

Human liver chimeric mice have become valuable tools for the study of human hepatotropic pathogens and for the investigation of metabolism and pharmacokinetics of novel drugs. The evolution of the underlying mouse models has been rapid in the past years. The diverse fields of applications of those model systems and their technical challenges will be discussed in this chapter.

Key words

Humanized mice Human liver chimeric mice Viral hepatitis Hepatitis C virus Hepatitis B virus Hepatitis delta virus Malaria DMPK 
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Notes

Acknowledgements

The authors thank Jenna Gaska for edits and critical discussion of the manuscript. Work in the laboratory is in part supported by grants from the National Institutes of Health (2 R01 AI079031-05A1, 1 R01 AI107301-01, 1 R56 AI106005-01, 1R21AI117213-01), a Scholar grant from the American Cancer Society (RSG-15-048-01-MPC), and the Grand Challenge Program of Princeton University. M.v.S. is a recipient of a fellowship from the German Research Foundation (Deutsche Forschungsgemeinschaft).

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Markus von Schaewen
    • 1
  • Gabriela Hrebikova
    • 1
  • Alexander Ploss
    • 1
  1. 1.Department of Molecular BiologyPrinceton UniversityPrincetonUSA

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