Protocols for Exosome Isolation and RNA Profiling

  • Fernando Marqués-GarcíaEmail author
  • María Isidoro-García
Part of the Methods in Molecular Biology book series (MIMB, volume 1434)


Exosomes are small extracellular vesicles of multivesicular bodies derived from the cell endosome. Molecules have a characteristic composition both in the membrane and in the carried load. These particles are located in the culture medium of cells in vitro and in vivo body fluids, being synthesized by almost all kind of cells. The most characteristic molecule transported within is the RNA with a large clinical potential. In this chapter, the methodology for isolation of exosomes and subsequent purification of the RNA is reviewed.

Key words

Biomarkers Exosome Extracellular vesicles Isolation Precipitation RNA profiling Ultracentrifugation 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Fernando Marqués-García
    • 1
    • 2
    Email author
  • María Isidoro-García
    • 1
    • 2
    • 3
  1. 1.Department of Clinical BiochemistryUniversity Hospital of SalamancaSalamancaSpain
  2. 2.Salamanca Institute for Biomedical Research (IBSAL)SalamancaSpain
  3. 3.Department of MedicineUniversity of SalamancaSalamancaSpain

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