Protocols for Exosome Isolation and RNA Profiling
Exosomes are small extracellular vesicles of multivesicular bodies derived from the cell endosome. Molecules have a characteristic composition both in the membrane and in the carried load. These particles are located in the culture medium of cells in vitro and in vivo body fluids, being synthesized by almost all kind of cells. The most characteristic molecule transported within is the RNA with a large clinical potential. In this chapter, the methodology for isolation of exosomes and subsequent purification of the RNA is reviewed.
Key wordsBiomarkers Exosome Extracellular vesicles Isolation Precipitation RNA profiling Ultracentrifugation
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