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Probing Chromosome Dynamics in Bacillus subtilis

  • Alan Koh
  • Heath Murray
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1431)

Abstract

Research over the last two decades has revealed that bacterial genomes are, in fact, highly organized. The goal of future research is to understand the molecular mechanisms underlying bacterial chromosome architecture and dynamics during the cell cycle. Here we discuss techniques that can be used with live cells to analyze chromosome structure and segregation in the gram-positive model organism Bacillus subtilis.

Key words

Microscopy Fluorescent protein Chromosome DNA replication DNA segregation Prokaryote 

Notes

Acknowledgement

This work was supported by a grant from the BBSRC (BB/K017527/1) and a Royal Society University Research Fellowship to H.M.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Centre for Bacterial Cell Biology, Institute for Cell & Molecular BiosciencesNewcastle UniversityNewcastle Upon TyneUK

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