Abstract
Application of optical mapping based on BioNano Genomics Irys® technology (http://www.bionanogenomics.com/) is growing rapidly since its debut in November 2012. The technology can be used to facilitate genome sequence assembly and analysis of genome structural variations. We describe here the detailed protocol that we used to generate a whole genome BioNano map for Aegilops tauschii, the D genome progenitor of hexaploid wheat (Triticum aestivum). We are using the whole genome BioNano map to validate sequence assembly based on the next-generation sequencing, order sequence scaffolds, and ultimately build pseudomolecules for the genome.
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Acknowledgement
This work was supported by the National Science Foundation grant IOS-1238231.
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Luo, MC. et al. (2016). Optical Nano-mapping and Analysis of Plant Genomes. In: Kianian, S., Kianian, P. (eds) Plant Cytogenetics. Methods in Molecular Biology, vol 1429. Humana Press, New York, NY. https://doi.org/10.1007/978-1-4939-3622-9_9
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DOI: https://doi.org/10.1007/978-1-4939-3622-9_9
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Publisher Name: Humana Press, New York, NY
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