Dendritic Cell Subset Purification from Human Tonsils and Lymph Nodes

  • Mélanie Durand
  • Elodie SeguraEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)


Dendritic cells (DCs) are a rare population of antigen-presenting cells that initiate immune responses in secondary lymphoid organs. In order to better understand the properties of DC in humans, it is essential to analyze DC subsets directly purified from tissues. Here, we describe a protocol allowing the purification of DC subsets from human tonsils and human lymph nodes.

Key words

Dendritic cells DC subsets Human Tonsil Lymph node 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institut Curie, Centre de rechercheParis Cedex 05France
  2. 2.INSERM U932Paris Cedex 05France

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