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Cross-Presentation Assay for Human Dendritic Cells

  • Elodie Segura
Part of the Methods in Molecular Biology book series (MIMB, volume 1423)

Abstract

The presentation of exogenous antigens on major histocompatibility complex (MHC) class I molecules, termed cross-presentation, is essential for the initiation of cytotoxic immune responses. Numerous studies in mice and human have shown that dendritic cells are the best cross-presenting cells. The protocol described here allows the assessment of the cross-presentation by human dendritic cells of a model antigen (either soluble or cell associated) to antigen-specific CD8 T cells.

Key words

Dendritic cells Cross-presentation Antigen processing Human CD8 T cells 

Notes

Acknowledgments

This work is supported by INSERM, the European Research Council, Labex DCBIOL, and Ligue contre le Cancer.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.INSERM U932, and Institut Curie, Centre de rechercheParis Cedex 05France

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