Characterization of Colorectal Cancer Development in Apcmin/+ Mice

  • ILKe NalbantogluEmail author
  • Valerie Blanc
  • Nicholas O. Davidson
Part of the Methods in Molecular Biology book series (MIMB, volume 1422)


The Apc min/+ mouse provides an excellent experimental model for studying genetic, environmental, and therapeutic aspects of intestinal neoplasia in humans. In this chapter, we will describe techniques for studying colon cancer development in Apc min/+ mice on C57BL/6J (B6) background, focusing on the roles of environmental modifiers, including Dextran Sulfate Sodium (DSS), high fat diet, and bile acid supplementation in the context of experimental colorectal cancer. This chapter also includes protocols describing extraction and purification of DSS-contaminated RNA, as well as sampling, harvesting, and tissue processing. The common pathologic lesions encountered in these animals are described in detail.

Key words

Apcmin/+ Colorectal cancer Adenoma Dextran sulfate sodium High fat Bile acids 



Work cited in this review was supported by the following grants: HL38180, DK56260 and Digestive Disease Research Core Center P30DK52574 to N.O.D.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • ILKe Nalbantoglu
    • 1
    Email author
  • Valerie Blanc
    • 2
  • Nicholas O. Davidson
    • 2
  1. 1.Department of Pathology and ImmunologyWashington University School of Medicine in St. LouisSt. LouisUSA
  2. 2.Department of MedicineWashington University School of Medicine in St. LouisSt. LouisUSA

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