An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?
The free radical nitric oxide (NO) is a universal signaling molecule among living organisms. To investigate versatile functions of NO in plants it is essential to analyze biologically produced NO with an appropriate method. Owing to the uniqueness of NO, plant researchers may encounter difficulties in applying methods that have been developed for mammalian study. Based on our experience, we present here a practical guide to NO measurement fitted to plant biology.
Key wordsChemiluminescence detection cPTIO DAF Electrochemical detection Nitric oxide RNS ROS RSS
Due to space limitations we were not able to cite many brilliant works on plant NO research that have applied the methods described in this chapter. Please refer to other chapters for such investigations. We thank Dr. Jon Fukuto for his valuable comments on this chapter. This work was supported by the grants to H.Y. from the Japanese Ministry of Education, Science, Culture and Sports.
- 3.Yamasaki H, Itoh RD, Bouchard JN et al (2011) Nitric oxide synthase-like activities in plants. In: Foyer CH, Zhang H (eds) Nitrogen metabolism in plants in the post-genomic era, vol 42, Annual Plant Reviews. Blackwell Publishing Ltd, West Sussex, pp 103–125Google Scholar
- 5.Yamasaki H (2004) Nitric oxide research in plant biology: its past and future. In: Magalhaes JR, Singh RP, Passos LP (eds) Nitric oxide signaling in higher plants. Focus on plant molecular biology. Studium Press, Houston, pp 1–23Google Scholar
- 39.Arita NO, Cohen MF, Tokuda G et al (2007) Fluorometric detection of nitric oxide with diaminofluoresceins (DAFs): applications and limitations for plant NO research. In: Lamattina L, Polacco J (eds) Nitric oxide in plant growth, development and stress physiology. Springer, Würzburg, pp 269–280CrossRefGoogle Scholar