An Overview of Methods in Plant Nitric Oxide (NO) Research: Why Do We Always Need to Use Multiple Methods?

  • Hideo YamasakiEmail author
  • Naoko S. Watanabe
  • Yasuko Sakihama
  • Michael F. Cohen
Part of the Methods in Molecular Biology book series (MIMB, volume 1424)


The free radical nitric oxide (NO) is a universal signaling molecule among living organisms. To investigate versatile functions of NO in plants it is essential to analyze biologically produced NO with an appropriate method. Owing to the uniqueness of NO, plant researchers may encounter difficulties in applying methods that have been developed for mammalian study. Based on our experience, we present here a practical guide to NO measurement fitted to plant biology.

Key words

Chemiluminescence detection cPTIO DAF Electrochemical detection Nitric oxide RNS ROS RSS 



Due to space limitations we were not able to cite many brilliant works on plant NO research that have applied the methods described in this chapter. Please refer to other chapters for such investigations. We thank Dr. Jon Fukuto for his valuable comments on this chapter. This work was supported by the grants to H.Y. from the Japanese Ministry of Education, Science, Culture and Sports.


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Hideo Yamasaki
    • 1
    Email author
  • Naoko S. Watanabe
    • 1
  • Yasuko Sakihama
    • 2
  • Michael F. Cohen
    • 3
    • 4
  1. 1.Faculty of ScienceUniversity of the RyukyusNishiharaJapan
  2. 2.Research Faculty of AgricultureHokkaido UniversitySapporoJapan
  3. 3.Department of BiologySonoma State UniversityRohnert ParkUSA
  4. 4.Biological Systems UnitOkinawa Institute of Science and TechnologyKunigami-gunJapan

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