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Extraction and Simultaneous Quantification of Endocannabinoids and Endocannabinoid-Like Lipids in Biological Tissues

  • Laura Bindila
  • Beat LutzEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1412)

Abstract

Extraction and quantification of endocannabinoids (eCBs) from biological tissues are essential to unravel their changes in physiological and pathophysiological conditions. We describe here an analytical protocol for extraction of endocannabinoids, anandamide (AEA) and 2-arachidonoyl glycerol (2-AG), endocannabinoid-like lipids such as palmitoyl ethanolamide (PEA) and oleoyl ethanolamide (OEA), as well as arachidonic acid (AA) from biological tissues using liquid-liquid extraction method and simultaneous quantification by liquid chromatography multiple reaction monitoring (LC/MRM).

Key words

Endocannabinoids Endocannabinoid-like lipids Liquid-chromatography Multiple reaction monitoring Lipid extraction 

Notes

Acknowledgments

This work has been financially supported by Collaborative Research Unit of the German Research Council FOR926 (central project CP1) and BMBF collaborative project LOGIN. We thank Claudia Schwitter, Raissa Lerner, and Julia Post for technical support in establishing the protocols.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.Institute for Physiological ChemistryUniversity Medical CenterMainzGermany

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