Visualizing Cancer Cell Chemotaxis and Invasion in 2D and 3D
We describe three chemotaxis assays—Insall chambers, circular invasion assays, and 3D organotypic assays—that are particularly appropriate for measuring migration of cancer cells in response to gradients of soluble attractants. Each assay has defined advantages, and together they provide the best possible quantitative assessment of cancer chemotaxis.
Key wordsChemotaxis Melanoma Metastasis Invasion Organotypic Cell migration
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