In Situ Assays of Chemotropism During Yeast Mating
Virtually all eukaryotic cells can grow in a polarized fashion in response to external signals. Cells can respond to gradients of chemoattractants or chemorepellents by directional growth, a process referred to as chemotropism. The budding yeast Saccharomyces cerevisiae undergoes chemotropic growth during mating, in which two haploid cells of opposite mating type grow towards one another. Mating pheromone gradients are essential for efficient mating in yeast and different yeast mutants are defective in chemotropism. Two methods of assessing the ability of yeast strains to respond to pheromone gradients are presented here.
Key wordsChemotropism Mating pheromone Bud scar Gradient Zygote
The authors would like to thank Edward Draper and Madhushalini Sukumar for images and for helpful discussion. This work was supported by the Centre National de la Recherche Scientifique (RAA), the Association pour la Recherche sur le Cancer (SFI20121205755) (RAA), the SIGNALIFE LabEX (RAA), and National Science Foundation (MCB1024718 and MCB-1415589) (DES).