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Vaccine Design pp 819-838 | Cite as

Preconditioning Vaccine Sites for mRNA-Transfected Dendritic Cell Therapy and Antitumor Efficacy

  • Kristen A. Batich
  • Adam M. Swartz
  • John H. SampsonEmail author
Part of the Methods in Molecular Biology book series (MIMB, volume 1403)

Abstract

Messenger RNA (mRNA)-transfected dendritic cell (DC) vaccines have been shown to be a powerful modality for eliciting antitumor immune responses in mice and humans; however, their application has not been fully optimized since many of the factors that contribute to their efficacy remain poorly understood. Work stemming from our laboratory has recently demonstrated that preconditioning the vaccine site with a recall antigen prior to the administration of a dendritic cell vaccine creates systemic recall responses and resultantly enhances dendritic cell migration to the lymph nodes with improved antitumor efficacy. This chapter describes the generation of murine mRNA-transfected DC vaccines, as well as a method for vaccine site preconditioning with protein antigen formulations that create potent recall responses.

Key words

Dendritic cells Bone marrow Transcription mRNA Electroporation Preconditioning Protein antigen formulation Intradermal 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Kristen A. Batich
    • 1
    • 2
  • Adam M. Swartz
    • 1
    • 2
  • John H. Sampson
    • 1
    • 2
    • 3
    • 4
    • 5
    Email author
  1. 1.Duke Brain Tumor Immunotherapy Program, Division of Neurosurgery, Department of SurgeryDuke University Medical CenterDurhamUSA
  2. 2.Department of PathologyDuke University Medical CenterDurhamUSA
  3. 3.Department of Radiation OncologyDuke University Medical CenterDurhamUSA
  4. 4.Department of ImmunologyDuke University Medical CenterDurhamUSA
  5. 5.The Preston Robert Tisch Brain Tumor CenterDuke University Medical CenterDurhamUSA

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