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Vaccine Design pp 763-777 | Cite as

Dendritic Cell Vaccines

  • Rachel Lubong SabadoEmail author
  • Marcia Meseck
  • Nina Bhardwaj
Part of the Methods in Molecular Biology book series (MIMB, volume 1403)

Abstract

Exploitation of the patient’s own immune system to induce antitumor immune responses using dendritic cell (DC) immunotherapy has been established in early clinical trials as a safe and promising therapeutic approach for cancer. However, their limited success in larger clinical trials highlights the need to optimize DC vaccine preparations. This chapter describes the methodologies utilized for the preparation of the DC vaccine most commonly used in clinical trials. Optional variations at different stages in DC vaccine preparation, based on the nature of antigen, delivery of antigen, maturation stimuli, and mode of administration for DC vaccines, are also presented for consideration as these are often dependent on the disease setting, desired immune response, and/or resources available.

Key words

Dendritic cell Vaccine Immunotherapy Immune response Tumor 

Notes

Acknowledgements

We would like to thank Andres Salazar for providing the poly-ICLC. We would also like to thank the staff of the Vaccine and Cell Therapy Laboratory—Farah Hasan, Hanqing Dong, Bike Su Oner, and Marina Aziz—for the help in refining the procedure.

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Rachel Lubong Sabado
    • 1
    Email author
  • Marcia Meseck
    • 1
  • Nina Bhardwaj
    • 1
  1. 1.Division of Hematology and Medical Oncology, Department of MedicineIcahn School of Medicine at Mount SinaiNew YorkUSA

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