Vaccine Design pp 739-751 | Cite as

Development of House Dust Mite Vaccine

Part of the Methods in Molecular Biology book series (MIMB, volume 1403)

Abstract

Mucosal vaccine based on lactic acid bacteria is an attractive strategy for prevention and treatment of allergic diseases. Here we describe the development of recombinant Lactococcus lactis expressing house dust mite (HDM) allergen as an oral vaccine. The major HDM allergen Der p2 is first codon optimized and synthesized to achieve the maximum expression level in L. lactis. After double digested by NcoI and XbaI, the derp2 fragment is ligated to the same double-digested pNZ8148 vector. The ligation is transformed to L. lactis NZ9000 and correct transformant is verified by sequencing. Western blot analysis is employed to confirm Derp2 expression in L. lactis after nisin induction.

Key words

Lactic acid bacteria House dust mite Allergy Mucosal vaccine 

References

  1. 1.
    Gregory LG, Lloyd CM (2011) Orchestrating house dust mite-associated allergy in the lung. Trends Immunol 32:402–411CrossRefPubMedPubMedCentralGoogle Scholar
  2. 2.
    Nelson RP Jr et al (1996) Allergen-specific IgE levels and mite allergen exposure in children with acute asthma first seen in an emergency department and in nonasthmatic control subjects. J Allergy Clin Immunol 98:258–263CrossRefPubMedGoogle Scholar
  3. 3.
    Thomas WR et al (2002) Characterization and immunobiology of house dust mite allergens. Int Arch Allergy Immunol 129:1–18CrossRefPubMedGoogle Scholar
  4. 4.
    Van Hage-Hamsten M, Valenta R (2002) Specific immunotherapy—the induction of new IgE-specificities? Allergy 57:375–378CrossRefPubMedGoogle Scholar
  5. 5.
    Dhanapala P, Doran T, Tang ML, Suphioglu C (2015) Production and immunological analysis of IgE reactive recombinant egg white allergens expressed in Escherichia coli. Mol Immunol 65:104–112CrossRefPubMedGoogle Scholar
  6. 6.
    Popovic M et al (2014) Yeast surface display is a novel tool for the rapid immunological characterization of plant-derived food allergens. Immunol Res 61:230–239CrossRefGoogle Scholar
  7. 7.
    Pahr S et al (2014) Biochemical, biophysical and IgE-epitope characterization of the wheat food allergen, Tri a 37. PLoS One 9:e111483CrossRefPubMedPubMedCentralGoogle Scholar
  8. 8.
    Huibregtse IL et al (2007) Induction of ovalbumin-specific tolerance by oral administration of Lactococcus lactis secreting ovalbumin. Gastroenterology 133:517–528CrossRefPubMedGoogle Scholar
  9. 9.
    Schwarzer M et al (2011) Neonatal colonization of mice with Lactobacillus plantarum producing the aeroallergen Bet v 1 biases towards Th1 and T-regulatory responses upon systemic sensitization. Allergy 66:368–375CrossRefPubMedGoogle Scholar
  10. 10.
    Zhang Q, Zhong J, Huan L (2011) Expression of hepatitis B virus surface antigen determinants in Lactococcus lactis for oral vaccination. Microbiol Res 166:111–120CrossRefPubMedGoogle Scholar
  11. 11.
    Ai C et al (2014) Genetically engineered Lactococcus lactis protect against house dust mite allergy in a BALB/c mouse model. PLoS One 9:e109461CrossRefPubMedPubMedCentralGoogle Scholar
  12. 12.
    Braat H et al (2006) A phase I trial with transgenic bacteria expressing interleukin-10 in Crohn’s disease. Clin Gastroenterol Hepatol 4:754–759CrossRefPubMedGoogle Scholar
  13. 13.
    Le Loir Y, Gruss A, Ehrlich SD, Langella P (1998) A nine-residue synthetic propeptide enhances secretion efficiency of heterologous proteins in Lactococcus lactis. J Bacteriol 180:1895–1903PubMedPubMedCentralGoogle Scholar

Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Food Science and TechnologyJiangnan UniversityWuxiPeople’s Republic of China

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