Vaccine Design pp 739-751 | Cite as

Development of House Dust Mite Vaccine

Part of the Methods in Molecular Biology book series (MIMB, volume 1403)


Mucosal vaccine based on lactic acid bacteria is an attractive strategy for prevention and treatment of allergic diseases. Here we describe the development of recombinant Lactococcus lactis expressing house dust mite (HDM) allergen as an oral vaccine. The major HDM allergen Der p2 is first codon optimized and synthesized to achieve the maximum expression level in L. lactis. After double digested by NcoI and XbaI, the derp2 fragment is ligated to the same double-digested pNZ8148 vector. The ligation is transformed to L. lactis NZ9000 and correct transformant is verified by sequencing. Western blot analysis is employed to confirm Derp2 expression in L. lactis after nisin induction.

Key words

Lactic acid bacteria House dust mite Allergy Mucosal vaccine 


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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  1. 1.School of Food Science and TechnologyJiangnan UniversityWuxiPeople’s Republic of China

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