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Facile Synthetic Access to Glycopeptide Antibiotic Precursor Peptides for the Investigation of Cytochrome P450 Action in Glycopeptide Antibiotic Biosynthesis

  • Clara Brieke
  • Veronika Kratzig
  • Madeleine Peschke
  • Max J. CryleEmail author
Protocol
Part of the Methods in Molecular Biology book series (MIMB, volume 1401)

Abstract

The glycopeptide antibiotics are an important class of complex, medically relevant peptide natural products. Given that the production of such compounds all stems from in vivo biosynthesis, understanding the mechanisms of the natural assembly system—consisting of a nonribosomal-peptide synthetase machinery (NRPS) and further modifying enzymes—is vital. In order to address the later steps of peptide biosynthesis, which are catalyzed by Cytochrome P450s that interact with the peptide-producing nonribosomal peptide synthetase, peptide substrates are required: these peptides must also be in a form that can be conjugated to carrier protein domains of the nonribosomal peptide synthetase machinery. Here, we describe a practical and effective route for the solid phase synthesis of glycopeptide antibiotic precursor peptides as their Coenzyme A (CoA) conjugates to allow enzymatic conjugation to carrier protein domains. This route utilizes Fmoc-chemistry suppressing epimerization of racemization-prone aryl glycine derivatives and affords high yields and excellent purities, requiring only a single step of simple solid phase extraction for chromatographic purification. With this, comprehensive investigations of interactions between various NRPS-bound substrates and Cytochrome P450s are enabled.

Key words

Glycopeptide antibiotics Solid phase peptide synthesis Coenzyme A Bio-conjugation Nonribosomal peptide synthetase Cytochrome P450 

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Copyright information

© Springer Science+Business Media New York 2016

Authors and Affiliations

  • Clara Brieke
    • 1
  • Veronika Kratzig
    • 1
  • Madeleine Peschke
    • 1
  • Max J. Cryle
    • 1
    Email author
  1. 1.Department of Biomolecular MechanismsMax Planck Institute for Medical ResearchHeidelbergGermany

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